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Anti-ENAH Antibody

144-65240
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetENAH
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-ENAH Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Gene ID55740
  • Target name
    ENAH
  • Target description
    ENAH actin regulator
  • Target synonyms
    ENA; enabled homolog; mammalian enabled variant 11a; mammalian enabled variant pan; MENA; NDPP1; protein enabled homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N8S7
  • Protein Name
    Protein enabled homolog
  • Scientific Description
    ENAH pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ENAH/MENA using anti-ENAH/MENA antibody (A05337-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse stomach tissue lysates, Lane 7: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENAH/MENA antigen affinity purified polyclonal antibody (Catalog # A05337-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ENAH/MENA at approximately 88 kDa. The expected band size for ENAH/MENA is at 67 kDa.
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