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Western Blot analysis of 293 cells using Mena Polyclonal Antibody
Western Blot analysis of 293 cells using Mena Polyclonal Antibody
Western Blot analysis of 293 cells using Mena Polyclonal Antibody

ENAH Antibody

CSB-PA030227
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetENAH
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Overview

  • Supplier
    Cusabio
  • Product Name
    ENAH Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55740
  • Target name
    ENAH
  • Target description
    ENAH actin regulator
  • Target synonyms
    ENA, MENA, NDPP1, protein enabled homolog, Mena delta 11a, enabled homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N8S7
  • Protein Name
    Protein enabled homolog
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ENAH/MENA using anti-ENAH/MENA antibody (A05337-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human SiHa whole cell lysates, Lane 5: rat stomach tissue lysates, Lane 6: mouse stomach tissue lysates, Lane 7: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENAH/MENA antigen affinity purified polyclonal antibody (Catalog # A05337-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ENAH/MENA at approximately 88 kDa. The expected band size for ENAH/MENA is at 67 kDa.
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