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Anti-EpCAM [AUA1]

Ab00609-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetEPCAM
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-EpCAM [AUA1]
  • Delivery Days Customer
    7
  • Application Supplier Note
    AUA1 cross reacts with breast carcinomas, kidney tubules, tonsil crypt epithelium and cells in some serious effusions. This is a good antibody for characterisation of all epithelial cells.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA; FACS; IHC; IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AUA1
  • Gene ID4072
  • Target name
    EPCAM
  • Target description
    epithelial cell adhesion molecule
  • Target synonyms
    Ber-Ep4, BerEp4, DIAR5, EGP-2, EGP314, EGP40, ESA, HNPCC8, KS1/4, KSA, LYNCH8, M4S1, MIC18, MK-1, MOC-31, TACSTD1, TROP1, epithelial cell adhesion molecule, adenocarcinoma-associated antigen, cell surface glycoprotein Trop-1, epithelial glycoprotein 314, human epithelial glycoprotein-2, major gastrointestinal tumor-associated protein GA733-2, membrane component, chromosome 4, surface marker (35kD glycoprotein), trophoblast cell surface antigen 1, tumor-associated calcium signal transducer 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP16422
  • Protein Name
    Epithelial cell adhesion molecule
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    LoVo cell line, a colon carcinoma cell line.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of EPCAM using anti-EPCAM antibody (PB10059). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPCAM antigen affinity purified polyclonal antibody (Catalog # PB10059) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPCAM at approximately 35-40 kDa. The expected band size for EPCAM is at 35 kDa.
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