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Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.

Anti-EPS15 Antibody

HPA008451
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetEPS15
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-EPS15
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2060
  • Target name
    EPS15
  • Target description
    epidermal growth factor receptor pathway substrate 15
  • Target synonyms
    AF-1P, AF1P, MLLT5, epidermal growth factor receptor substrate 15, ALL1 fused gene from chromosome 1, protein AF-1p
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42566
  • Protein Name
    Epidermal growth factor receptor substrate 15
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of EPS15 using anti-EPS15 antibody (A01681-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse NIH-3T3 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPS15 antigen affinity purified polyclonal antibody (Catalog # A01681-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPS15 at approximately 138KD. The expected band size for EPS15 is at 138KD.
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