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Western Blot analysis of JK cells using Phospho-Eps15 (Y849) Polyclonal Antibody
Western Blot analysis of JK cells using Phospho-Eps15 (Y849) Polyclonal Antibody
Western Blot analysis of JK cells using Phospho-Eps15 (Y849) Polyclonal Antibody

Phospho-EPS15 (Y849) Antibody

CSB-PA008076
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetEPS15
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Overview

  • Supplier
    Cusabio
  • Product Name
    Phospho-EPS15 (Y849) Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2060
  • Target name
    EPS15
  • Target description
    epidermal growth factor receptor pathway substrate 15
  • Target synonyms
    AF-1P, AF1P, MLLT5, epidermal growth factor receptor substrate 15, ALL1 fused gene from chromosome 1, protein AF-1p
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42566
  • Protein Name
    Epidermal growth factor receptor substrate 15
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EPS15 using anti-EPS15 antibody (A01681-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: mouse NIH-3T3 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: human PC-3 whole cell lysates, Lane 8: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPS15 antigen affinity purified polyclonal antibody (Catalog # A01681-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPS15 at approximately 138KD. The expected band size for EPS15 is at 138KD.
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