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Anti-ERVW-1 Antibody

144-64458
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetERVW-1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-ERVW-1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID30816
  • Target name
    ERVW-1
  • Target description
    endogenous retrovirus group W member 1, envelope
  • Target synonyms
    ENV, ENVW, ERVWE1, HERV-7q, HERV-W-ENV, HERV7Q, HERVW, HERVWENV, syncytin-1, HERV-7q envelope protein, HERV-W Env glycoprotein, HERV-W envelope protein, HERV-W_7q21.2 provirus ancestral Env polyprotein, HERV-tryptophan envelope protein, endogenous retroviral family W, env(C7), member 1, endogenous retrovirus group W, member 1, envelope glycoprotein, envelope polyprotein gPr73, enverin, human endogenous retrovirus W envC7-1 envelope protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UQF0
  • Protein Name
    Syncytin-1
  • Scientific Description
    ERVW-1 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ERVW-1 using anti-ERVW-1 antibody (PB9622). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERVW-1 antigen affinity purified polyclonal antibody (Catalog # PB9622) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERVW-1 at approximately 80 kDa. The expected band size for ERVW-1 is at 80 kDa.
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