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The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA215261(ERVW-1 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA215261(ERVW-1 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using CSB-PA215261(ERVW-1 Antibody) at dilution 1/45, on the right is treated with synthetic peptide. (Original magnification: x200)

ERVW-1 Antibody

CSB-PA215261
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetERVW-1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ERVW-1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID30816
  • Target name
    ERVW-1
  • Target description
    endogenous retrovirus group W member 1, envelope
  • Target synonyms
    endogenous retroviral family W, env(C7), member 1; endogenous retrovirus group W, member 1; ENV; envelope glycoprotein; envelope polyprotein gPr73; enverin; ENVW; ERVWE1; HERV7Q; HERV-7q; HERV-7q envelope protein; HERV-tryptophan envelope protein; HERVW; HERV-W Env glycoprotein; HERV-W envelope protein; HERV-W_7q21.2 provirus ancestral Env polyprotein; HERVWENV; HERV-W-ENV; human endogenous retrovirus W envC7-1 envelope protein; syncytin-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UQF0
  • Protein Name
    Syncytin-1
  • Scientific Description
    Many different human endogenous retrovirus (HERV) families are expressed in normal placental tissue at high levels, suggesting that HERVs are functionally important in reproduction. This gene is part of an HERV provirus on chromosome 7 that has inactivating mutations in the gag and pol genes. This gene is the envelope glycoprotein gene which appears to have been selectively preserved. The genes protein product is expressed in the placental syncytiotrophoblast and is involved in fusion of the cytotrophoblast cells to form the syncytial layer of the placenta.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ERVW-1 using anti-ERVW-1 antibody (PB9622). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERVW-1 antigen affinity purified polyclonal antibody (Catalog # PB9622) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ERVW-1 at approximately 80 kDa. The expected band size for ERVW-1 is at 80 kDa.
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