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Anti-estrogen receptor beta [CO1531]

AB02568-10.0-BT
Absolute Antibody
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetESR2
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-estrogen receptor beta [CO1531]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody was used for western blot on tissues of C57BL/6J wild-type mice (Montani et al, 2008; pmid:18281260). Whole-cell extracts from cells expressing ERalpha-LBD or full-length ERbeta were immunoprecipitated with this antibody (Leong et al, 2005; pmid:16051668).
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CO1531
  • Gene ID2100
  • Target name
    ESR2
  • Target description
    estrogen receptor 2
  • Target synonyms
    ER-BETA, ESR-BETA, ESRB, ESTRB, Erb, NR3A2, ODG8, estrogen receptor beta, estrogen receptor beta 2, estrogen receptor beta 4, nuclear receptor subfamily 3 group A member 2, oestrogen receptor beta
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDQ92731
  • Protein Name
    Estrogen receptor beta
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ESR2 using anti-ESR2 antibody (A00786-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESR2 antigen affinity purified polyclonal antibody (Catalog # A00786-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESR2 at approximately 59 kDa. The expected band size for ESR2 is at 59 kDa.
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