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Immunohistochemical staining of human ovary shows moderate nuclear positivity in follicle cells.
Immunohistochemical staining of human ovary shows moderate nuclear positivity in follicle cells.
Immunohistochemical staining of human ovary shows moderate nuclear positivity in follicle cells.

Anti-EZH1 Antibody

HPA077684
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetEZH1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-EZH1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2145
  • Target name
    EZH1
  • Target description
    enhancer of zeste 1 polycomb repressive complex 2 subunit
  • Target synonyms
    KMT6B, histone-lysine N-methyltransferase EZH1, ENX-2, enhancer of zeste homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92800
  • Protein Name
    Histone-lysine N-methyltransferase EZH1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of EZH1 using anti-EZH1 antibody (A02494-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: human Hacat whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EZH1 antigen affinity purified polyclonal antibody (Catalog # A02494-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EZH1 at approximately 100 kDa, 60 kDa. The expected band size for EZH1 is at 68 kDa.
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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EZH1 antibody [N1], N-term (GTX108013) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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