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Western Blot analysis of COS7 cells using ENX-2 Polyclonal Antibody
Western Blot analysis of COS7 cells using ENX-2 Polyclonal Antibody
Western Blot analysis of COS7 cells using ENX-2 Polyclonal Antibody

EZH1 Antibody

CSB-PA002349
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetEZH1
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Overview

  • Supplier
    Cusabio
  • Product Name
    EZH1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2145
  • Target name
    EZH1
  • Target description
    enhancer of zeste 1 polycomb repressive complex 2 subunit
  • Target synonyms
    KMT6B, histone-lysine N-methyltransferase EZH1, ENX-2, enhancer of zeste homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92800
  • Protein Name
    Histone-lysine N-methyltransferase EZH1
  • Reactivity
    Human, Monkey, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of EZH1 using anti-EZH1 antibody (A02494-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: human Hacat whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 7: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EZH1 antigen affinity purified polyclonal antibody (Catalog # A02494-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EZH1 at approximately 100 kDa, 60 kDa. The expected band size for EZH1 is at 68 kDa.
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