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Anti-EZR [SAIC-37C-23]

Ab00330-1.1
Absolute Antibody
ApplicationsMass Spectrometry, Western Blot
Product group Antibodies
ReactivityHuman
TargetEZR
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-EZR [SAIC-37C-23]
  • Delivery Days Customer
    7
  • Application Supplier Note
    Original data characterizing this antibody may be found here .
  • Applications
    Mass Spectrometry, Western Blot
  • Applications Supplier
    WB; immunoMRM
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SAIC-37C-23
  • Gene ID7430
  • Target name
    EZR
  • Target description
    ezrin
  • Target synonyms
    CVIL, CVL, HEL-S-105, VIL2, ezrin, cytovillin 2, epididymis secretory protein Li 105, p81, villin 2 (ezrin)
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP15311
  • Protein Name
    Ezrin
  • Scientific Description
    This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The KLH-conjugated peptide "SGYLSSER" derived from Ezrin, a protein likely involved in connections of major cytoskeletal structures to the plasma membrane.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of Ezrin/EZR using anti-Ezrin/EZR antibody (A01750-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ezrin/EZR antigen affinity purified polyclonal antibody (Catalog # A01750-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ezrin/EZR at approximately 81 kDa. The expected band size for Ezrin/EZR is at 69 kDa.
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