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Figure 1. Western blotting validation for Anti-Ezrin (phospho-Y353) Antibody A01750Y353 Western blot (WB) analysis of p-Ezrin (Y353) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:PMVEC whole cell lysate(40ug) Lane4:3T3-L1 whole cell lysate(40ug) Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Figure 1. Western blotting validation for Anti-Ezrin (phospho-Y353) Antibody A01750Y353 Western blot (WB) analysis of p-Ezrin (Y353) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:PMVEC whole cell lysate(40ug) Lane4:3T3-L1 whole cell lysate(40ug) Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Figure 1. Western blotting validation for Anti-Ezrin (phospho-Y353) Antibody A01750Y353 Western blot (WB) analysis of p-Ezrin (Y353) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:PMVEC whole cell lysate(40ug) Lane4:3T3-L1 whole cell lysate(40ug) Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Anti-Ezrin (phospho-Y353) Antibody

A01750Y353
Boster Bio
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetEZR
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Ezrin (phospho-Y353) Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID7430
  • Target name
    EZR
  • Target description
    ezrin
  • Target synonyms
    CVIL, CVL, HEL-S-105, VIL2, ezrin, cytovillin 2, epididymis secretory protein Li 105, p81, villin 2 (ezrin)
  • Host
    Rabbit
  • Protein IDP15311
  • Protein Name
    Ezrin
  • Scientific Description
    Boster Bio Anti-Ezrin (phospho-Y353) Antibody catalog # A01750Y353. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ezrin/EZR using anti-Ezrin/EZR antibody (A01750-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Hela whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: human Daudi whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ezrin/EZR antigen affinity purified polyclonal antibody (Catalog # A01750-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ezrin/EZR at approximately 81 kDa. The expected band size for Ezrin/EZR is at 69 kDa.
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