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ProductsBack/Anti-Factor VIII A2 domain [BOIIB2]

Anti-Factor VIII A2 domain [BOIIB2]

AB03957-10.0-BT
Absolute Antibody
ApplicationsELISA, Neutralisation/Blocking
Product group Antibodies
ReactivityHuman
TargetF8
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Factor VIII A2 domain [BOIIB2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The binding specificity and epitope characterization of this antibody was determined using ELISA. The ability of this antibody inhibit factor VIII function in vitro was studied using a chromogenic functional assay. It was reported that this antibody inhibited the conversion of factor X to factor Xa at concentration of 0.1 microg/ml. It was found that this antibody inhibits thrombin production using both the intrinsic and extrinsic pathway of coagulation (US20080206254).
  • Applications
    ELISA, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BOIIB2
  • Gene ID2157
  • Target name
    F8
  • Target description
    coagulation factor VIII
  • Target synonyms
    AHF, DXS1253E, F8B, F8C, FVIII, HEMA, THPH13, coagulation factor VIII, antihemophilic factor, coagulation factor VIII A1 domain, coagulation factor VIII C2 domain, coagulation factor VIII, procoagulant component, coagulation factor VIIIc, factor VIII F8B
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP00451
  • Protein Name
    Coagulation factor VIII
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Factor VIII using anti-Factor VIII antibody (PA2061). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse lung tissue lysates, Lane 6: human Hela whole cell lysates, Lane 7: human HepG2 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Factor VIII antigen affinity purified polyclonal antibody (Catalog # PA2061) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Factor VIII at approximately 92KD. The expected band size for Factor VIII is at 92KD.
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