Anti-Factor VIII [KM33]

Absolute Antibody

Anti-Factor VIII [KM33]

9

This antibody binds the C1 domain of the human factor VIII, a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for factor IXa which converts factor X to the activated form Xa in the presence of calcium and phospholipids. Defects

The scFv version of this antibody was capable of inhibiting factor VIII activity with inhibition titre of 97 BU/mg. The scFv antibody binds human factor VIII light chain with a binding affinity of Kd= 0.1 nM. It was reported that scFv KM33 inhibits the procoagulant activity of factor VIII, although its epitope is located outside region R1803-K1818 (PMID: 11159524). It was reported that the presence of this antibody completely inhibited FVIII endocytosis by both monocyte and bone marrow derived mouse dendritic cells (PMID: 21962992). It was reported that this antibody inhibits FVIII activity and interactions with its physiological ligands, von Willebrand factor, phospholipids and catabolic receptors in vitro (PMID: 11159524; Limburg V et al., 2005).

Functional Assay, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

KM33

F8

coagulation factor VIII

AHF; antihemophilic factor; coagulation factor VIII; coagulation factor VIII A1 domain; coagulation factor VIII C2 domain; coagulation factor VIII, procoagulant component; coagulation factor VIIIc; DXS1253E; F8B; F8C; factor VIII F8B; FVIII; HEMA

Mouse

IgG1

Coagulation factor VIII

Human

-20°C,2°C to 8°C

12352203