Anti-Factor VIII [KM41]

Absolute Antibody

Anti-Factor VIII [KM41]

9

This antibody binds and epitope between amino acid R1803-K1818 of the A3-C1 domains of the human factor VIII, which also contain binding site for factor IXa. Factor VIII is a glycoprotein involved in blood coagulation. Factor VIII acts as a cofactor for f

The scFv version of this antibody was capable of inhibiting factor VIII activity with inhibition titre of 63 BU/mg. The scFv antibody binds human factor VIII light chain with a binding affinity of Kd= 1.6 nM (PMID: 11159524). It was reported that scFv KM33 inhibits the procoagulant activity of factor VIII. In view of the similar epitope specificity of scFv KM41 and CLB-CAg A (antibody which binds a synthetic peptide comprising residues R1803-K1818), it is likely that scFv KM41 competes with factor IXa for binding to the factor VIII light chain (PMID: 11159524).

Functional Assay, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

KM41

F8

coagulation factor VIII

AHF; antihemophilic factor; coagulation factor VIII; coagulation factor VIII A1 domain; coagulation factor VIII C2 domain; coagulation factor VIII, procoagulant component; coagulation factor VIIIc; DXS1253E; F8B; F8C; factor VIII F8B; FVIII; HEMA

Human

IgG1

Coagulation factor VIII

This is a reformatted human IgG1 Fc Silent™ antibody, based on the original human scFv format, created for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203