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Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.

Anti-FAM129B Antibody

HPA021417
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNIBAN2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FAM129B
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64855
  • Target name
    NIBAN2
  • Target description
    niban apoptosis regulator 2
  • Target synonyms
    bA356B19.6; C9orf88; FAM129B; family with sequence similarity 129 member B; MEG-3; melanoma invasion by ERK; MINERVA; niban-like protein 1; OC58; protein Niban 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96TA1
  • Protein Name
    Niban-like protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FAM129B/NIBAN2 using anti-FAM129B/NIBAN2 antibody (A09391-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse stomach tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM129B/NIBAN2 antigen affinity purified polyclonal antibody (Catalog # A09391-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAM129B/NIBAN2 at approximately 95 kDa. The expected band size for FAM129B/NIBAN2 is at 95 kDa.
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