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Immunohistochemical staining of human kidney shows strong nucleolar positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong nucleolar positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong nucleolar positivity in cells in tubules.

Anti-FAM60A Antibody

HPA057585
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSINHCAF
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FAM60A Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID58516
  • Target name
    SINHCAF
  • Target description
    SIN3-HDAC complex associated factor
  • Target synonyms
    C12orf14, FAM60A, L4, TERA, SIN3-HDAC complex-associated factor, family with sequence similarity 60 member A, protein FAM60A, tera protein homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NP50
  • Protein Name
    SIN3-HDAC complex-associated factor
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SINHCAF using anti-SINHCAF antibody (A31944-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SINHCAF antigen affinity purified polyclonal antibody (Catalog # A31944-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SINHCAF at approximately 28-30 kDa. The expected band size for SINHCAF is at 25 kDa.
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ReactivityHuman, Rat
TargetSINHCAF
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