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Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA882059LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA882059LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA882059LA01HU at dilution of 1:100

FAM60A Antibody

CSB-PA882059LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSINHCAF
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Overview

  • Supplier
    Cusabio
  • Product Name
    FAM60A Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID58516
  • Target name
    SINHCAF
  • Target description
    SIN3-HDAC complex associated factor
  • Target synonyms
    C12orf14, FAM60A, L4, TERA, SIN3-HDAC complex-associated factor, family with sequence similarity 60 member A, protein FAM60A, tera protein homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NP50
  • Protein Name
    SIN3-HDAC complex-associated factor
  • Scientific Description
    Subunit of the Sin3 deacetylase complex (Sin3/HDAC), this subunit is important for the repression of genes encoding components of the TGF-beta signaling pathway.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SINHCAF using anti-SINHCAF antibody (A31944-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SINHCAF antigen affinity purified polyclonal antibody (Catalog # A31944-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SINHCAF at approximately 28-30 kDa. The expected band size for SINHCAF is at 25 kDa.
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TargetSINHCAF
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