Anti-FAP [3F2]

Absolute Antibody

Anti-FAP [3F2]

7

This antibody can bind human, mouse and cynomolgus monkey fibroblast activation protein.

The original mouse antibody was used to check the expression levels of FAP on the surface of GM05389 lung fibroblasts using flow cytometry. The original mouse IgG1 version of this antibody binds human, mouse and cynomolgus monkey FAP with an avidity of 39 pM, 29 pM and 42 pM respectively. Binding of human IgG1 antibody 3F2 to human and murine FAP expressed on stably transfected HEK293 cells, human fibroblasts cell line GM05389 was measured by floy cytometry. The mouse IgG2a version of this antibody was also used for the immunohistochemical stainings of frozen tissue microarray (TMA) containing 10 invasive ductal carcinomas of the breast, 10 colorectal adenocarcinomas and 10 non-small cell lung cancers (US20200079873).

Flow Cytometry, ELISA, ImmunoHistoChemistry

FC; ELISA; IHC

Research Use Only

Monoclonal

3F2

FAP

fibroblast activation protein alpha

170 kDa melanoma membrane-bound gelatinase; dipeptidyl peptidase FAP; DPPIV; FAPA; FAPalpha; gelatine degradation protease FAP; integral membrane serine protease; post-proline cleaving enzyme; prolyl endopeptidase FAP; seprase; SIMP; surface-expressed protease

Human

IgG1

Prolyl endopeptidase FAP

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Human, Monkey, Mouse

Human, mouse, cynomolgus monkey

-20°C,2°C to 8°C

12352203