Bio-Connect

Anti-FAP [73.3]

Research Use Only
Ab03229-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, Other Application
Product group Antibodies
ReactivityMouse
TargetFap
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-FAP [73.3]
  • Delivery Days Customer
    7
  • Antibody Specificity
    The antibody is specific for fibroblast activation protein (FAP). FAP is a membrane protease that is highly expressed by cancer-associated fibroblasts (CAFs).
  • Application Supplier Note
    A second-generation retroviral CAR, consisting of the scFv fragment of the antibody, with CD8 stalk, human CD3aeta and 4-1BB domains, targeting mouse FAP was developed. The Fap-CAR-T cells killed FAP-expressing 3T3 target cells specifically. The FAP-CAR-T cells were tested in vivo in three different established models, showing that the growth of tumors was significantly reduced by 35-50% following treatment. Further, the anti-FAP-CAR T cells demonstrated enhanced antitumor response when combined with a tumor vaccine. No significant toxicity was observed in the treated mice. (Wang et al., 2014; PMID: 24778279) FAP+ cells were identified using flow cytometry of lung tissue using the antibody (Kimura et al., 2019; PMID: 31188013). This antibody was used for detection of murine FAP expression on HT1080 cells by flow cytometry (de Sostoa et al. 2019, PMID: 30683154). Immunoprecipitation of FAP was performed on lung humogenate using this antibody. The antibody detected FAP by western blot analysis (Fan et al., 2016; PMID: 26663085).
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, Other Application
  • Applications Supplier
    WB; IP; FC; in vitro; in vivo
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    73.3
  • Gene ID14089
  • Target name
    Fap
  • Target description
    fibroblast activation protein
  • Target synonyms
    dipeptidyl peptidase FAP; FAPalpha; fibroblast activation protein alpha; gelatine degradation protease FAP; integral membrane serine protease; post-proline cleaving enzyme; prolyl endopeptidase FAP; seprase; serine integral membrane protease; SIMP; surface-expressed protease
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP97321
  • Protein Name
    Prolyl endopeptidase FAP
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Reactivity Supplier Note
    The original antibody was generated by immunizing FAP-null mice with FAP-expressing 3T3 cells.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203