Anti-FAP [73.3]

Absolute Antibody

Anti-FAP [73.3]

7

A second-generation retroviral CAR, consisting of the scFv fragment of the antibody, with CD8 stalk, human CD3aeta and 4-1BB domains, targeting mouse FAP was developed. The Fap-CAR-T cells killed FAP-expressing 3T3 target cells specifically. The FAP-CAR-T cells were tested in vivo in three different established models, showing that the growth of tumors was significantly reduced by 35-50% following treatment. Further, the anti-FAP-CAR T cells demonstrated enhanced antitumor response when combined with a tumor vaccine. No significant toxicity was observed in the treated mice. (Wang et al., 2014; PMID: 24778279) FAP+ cells were identified using flow cytometry of lung tissue using the antibody (Kimura et al., 2019; PMID: 31188013). This antibody was used for detection of murine FAP expression on HT1080 cells by flow cytometry (de Sostoa et al. 2019, PMID: 30683154). Immunoprecipitation of FAP was performed on lung humogenate using this antibody. The antibody detected FAP by western blot analysis (Fan et al., 2016; PMID: 26663085).

Flow Cytometry, ImmunoPrecipitation, Western Blot, Other Application

WB; IP; FC; in vitro; in vivo

Research Use Only

Monoclonal

73.3

Fap

fibroblast activation protein

dipeptidyl peptidase FAP; FAPalpha; fibroblast activation protein alpha; gelatine degradation protease FAP; integral membrane serine protease; post-proline cleaving enzyme; prolyl endopeptidase FAP; seprase; serine integral membrane protease; SIMP; surface-expressed protease

Mouse

IgG1

Prolyl endopeptidase FAP

Mouse

The original antibody was generated by immunizing FAP-null mice with FAP-expressing 3T3 cells.

-20°C,2°C to 8°C

12352203