Anti-Fas [R-125224]

Absolute Antibody

Anti-Fas [R-125224]

9

R-125224 binds to the extracellular portion of human Fas at an eptiope consisting of the sequence RTQNTKCRCK (aa 105-114) (pmid: 11754745). Fas is a type I membrane protein which belongs to the tumor necrosis factor (TNF) receptor/nerve growth factor (NGF

R-125224 shows the same binding affinity and the same ability to induce apoptosis in WR19L12a cells that express human Fas as the parental murine HFE7A antibody. R-125224 selectively induces apoptosis in type I activated lymphocytes but not in type II cells. R-125224 is able to induce apoptosis in the human lymphoid cell lines H9 and SKW6.4, as well as activated human lymphocytes, when cross-linked with anti-hIgG secondary antibodies. The antibody is unable to induce apoptosis in HPB-ALL cells, Jurkat cells or human hepatocytes. R-125224 has been used in vivo where it has been shown to greatly reduce the number of activated human human CD3+ Fas+ T cells in a SCID mouse model possessing a functional human immune system. Fas antigen tissue distribution in cynomolgus monkeys with collagen-induced arthritis at the arm joint (CIA monkeys) has been studied using [125I]-Labeled R-125224. High radioactivity in the bone marrow, thymus, lungs, liver, adrenals, spleen, ovaries, axillary lymph node and mesenteric lymph node compared to the radioactivity in the plasma was observed, which correlates with Fas expression. Fas can also be detected by R-125224 by ELISA.

Flow Cytometry, ELISA, Other Application

Research Use Only

Monoclonal

R-125224

FAS

Fas cell surface death receptor

ALPS1A; APO-1; APO-1 cell surface antigen; apoptosis antigen 1; apoptosis signaling receptor FAS; apoptosis-mediating surface antigen FAS; APT1; CD95; CD95 antigen; Fas (TNF receptor superfamily, member 6); Fas AMA; FAS1; FASLG receptor; FASTM; mutant tumor necrosis receptor superfamily member 6; TNF receptor superfamily member 6; TNFRSF6; tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6

Human

IgM

Tumor necrosis factor receptor superfamily member 6

This reformatted human antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203