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Immunohistochemical staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate to strong cytoplasmic positivity in hepatocytes.

Anti-FBP1 Antibody

HPA012513
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFBP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FBP1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2203
  • Target name
    FBP1
  • Target description
    fructose-bisphosphatase 1
  • Target synonyms
    FBP, fructose-1,6-bisphosphatase 1, D-fructose-1,6-bisphosphate 1-phosphohydrolase 1, FBPase 1, growth-inhibiting protein 17, liver FBPase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09467
  • Protein Name
    Fructose-1,6-bisphosphatase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of FBP1 using anti-FBP1 antibody (A01377-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBP1 antigen affinity purified polyclonal antibody (Catalog # A01377-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FBP1 at approximately 37 kDa. The expected band size for FBP1 is at 37 kDa.
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