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Western blot All lanes: FBP1 antibody at 3.06ug/ml Lane 1: Mouse liver tissue Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 37 kDa Observed band size: 37 kDa
Western blot All lanes: FBP1 antibody at 3.06ug/ml Lane 1: Mouse liver tissue Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 37 kDa Observed band size: 37 kDa
Western blot All lanes: FBP1 antibody at 3.06ug/ml Lane 1: Mouse liver tissue Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 37 kDa Observed band size: 37 kDa

FBP1 Antibody

CSB-PA008459ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetFBP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    FBP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2203
  • Target name
    FBP1
  • Target description
    fructose-bisphosphatase 1
  • Target synonyms
    FBP, fructose-1,6-bisphosphatase 1, D-fructose-1,6-bisphosphate 1-phosphohydrolase 1, FBPase 1, growth-inhibiting protein 17, liver FBPase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09467
  • Protein Name
    Fructose-1,6-bisphosphatase 1
  • Scientific Description
    Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate in the presence of divalent cations, acting as a rate-limiting enzyme in gluconeogenesis. Plays a role in regulating glucose sensing and insulin secretion of pancreatic beta-cells. Appears to modulate glycerol gluconeogenesis in liver. Important regulator of appetite and adiposity; increased expression of the protein in liver after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus seems to provide a feedback mechanism to limit weight gain.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FBP1 using anti-FBP1 antibody (A01377-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FBP1 antigen affinity purified polyclonal antibody (Catalog # A01377-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FBP1 at approximately 37 kDa. The expected band size for FBP1 is at 37 kDa.
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