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ProductsBack/Anti-FcRn [HL161-11G]

Anti-FcRn [HL161-11G]

AB02467-10.0-BT
Absolute Antibody
ApplicationsFlow Cytometry, Other Application
Product group Antibodies
TargetFCGRT
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-FcRn [HL161-11G]
  • Delivery Days Customer
    9
  • Application Supplier Note
    Surface plasmon resonance was performed to determine how strongly this antibody binds to soluble hFcRn (KR20130071961). Flow cytometery was performed on HEK293 cells expressing human FcRn, 3T3-L1 cells stably expressing mouse FcRn, Rat-2, a rat fibroblast stably expressing rat FcRn and Cos7 cells stably expressing monkey FcRn. The flow cytometry experiment used this antibody (KR20130071961).
  • Applications
    Flow Cytometry, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL161-11G
  • Gene ID2217
  • Target name
    FCGRT
  • Target description
    Fc fragment of IgG receptor and transporter
  • Target synonyms
    alpha-chain; Fc fragment of IgG, receptor, transporter, alpha; FCRN; FcRn alpha chain; heavy chain of the major histocompatibility complex class I-like Fc receptor; IgG Fc fragment receptor transporter alpha chain; IgG receptor FcRn large subunit p51; immunoglobulin receptor, intestinal, heavy chain; major histocompatibility complex class I-like Fc receptor; neonatal Fc receptor; neonatal Fc-receptor for Ig; transmembrane alpha chain of the neonatal receptor
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP55899
  • Protein Name
    IgG receptor FcRn large subunit p51
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FCGRT using anti-FCGRT antibody (PB9866). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCGRT antigen affinity purified polyclonal antibody (Catalog # PB9866) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FCGRT at approximately 50 kDa. The expected band size for FCGRT is at 40 kDa.
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