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Western blot All lanes: FCGRT antibody at 2microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Mouse liver tissue Lane 3: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa
Western blot All lanes: FCGRT antibody at 2microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Mouse liver tissue Lane 3: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa
Western blot All lanes: FCGRT antibody at 2microg/ml Lane 1: HepG2 whole cell lysate Lane 2: Mouse liver tissue Lane 3: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40 kDa Observed band size: 40 kDa

FCGRT Antibody

CSB-PA008545EA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetFCGRT
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Overview

  • Supplier
    Cusabio
  • Product Name
    FCGRT Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2217
  • Target name
    FCGRT
  • Target description
    Fc gamma receptor and transporter
  • Target synonyms
    FCRN, FcgammaRn, alpha-chain, IgG receptor FcRn large subunit p51, Fc fragment of IgG receptor and transporter, Fc fragment of IgG, receptor, transporter, alpha, FcRn alpha chain, IgG Fc fragment receptor transporter alpha chain, heavy chain of the major histocompatibility complex class I-like Fc receptor, immunoglobulin receptor, intestinal, heavy chain, major histocompatibility complex class I-like Fc receptor, neonatal Fc receptor, neonatal Fc-receptor for Ig, neonatal fragment crystallizable Fc receptor FcRn, transmembrane alpha chain of the neonatal receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55899
  • Protein Name
    IgG receptor FcRn large subunit p51
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FCGRT using anti-FCGRT antibody (PB9866). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCGRT antigen affinity purified polyclonal antibody (Catalog # PB9866) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FCGRT at approximately 50 kDa. The expected band size for FCGRT is at 40 kDa.
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