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Immunohistochemical staining of human tonsil shows moderate nuclear positivity in lymphoid cells outside reaction center.
Immunohistochemical staining of human tonsil shows moderate nuclear positivity in lymphoid cells outside reaction center.
Immunohistochemical staining of human tonsil shows moderate nuclear positivity in lymphoid cells outside reaction center.

Anti-FER Antibody

HPA007641
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFER
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FER Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2241
  • Target name
    FER
  • Target description
    FER tyrosine kinase
  • Target synonyms
    PPP1R74, TYK3, p94-Fer, tyrosine-protein kinase Fer, feline encephalitis virus-related kinase FER, fer (fps/fes related) tyrosine kinase, fujinami poultry sarcoma/Feline sarcoma-related protein Fer, phosphoprotein NCP94, protein phosphatase 1, regulatory subunit 74, proto-oncogene c-Fer, tyrosine kinase 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16591
  • Protein Name
    Tyrosine-protein kinase Fer
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of FER using anti-FER antibody (A01630-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FER antigen affinity purified polyclonal antibody (Catalog # A01630-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FER at approximately 95 kDa. The expected band size for FER is at 95 kDa.
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