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Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Anti-FGFR4 Antibody

HPA027369
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFGFR4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FGFR4
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2264
  • Target name
    FGFR4
  • Target description
    fibroblast growth factor receptor 4
  • Target synonyms
    CD334, JTK2, TKF, fibroblast growth factor receptor 4, hydroxyaryl-protein kinase, protein-tyrosine kinase, tyrosine kinase related to fibroblast growth factor receptor, tyrosylprotein kinase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22455
  • Protein Name
    Fibroblast growth factor receptor 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FGFR4 using anti-FGFR4 antibody (PB9193). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human FGFR4 protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR4 antigen affinity purified polyclonal antibody (Catalog # PB9193) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FGFR4 at approximately 39 kDa. The expected band size for FGFR4 is at 39 kDa.
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