Anti-Fibronectin [C6], Mouse IgG1, kappa

Absolute Antibody

Anti-Fibronectin [C6], Mouse IgG1, kappa

9

The original version of this antibody (mouse IgG1) was generated and used in ELISA, WB, IHC, SPR, and in vivo assays. ELISA analysis showed that the antibody reacts with type-III-repeats-containing FN fragments 7.EDB.8.9 and 7.EDB, and a Kd of 10 nM was measured in an SPR assay with 7.EDB.8.9 as the antigen. Western blot analysis showed that the epitope recognized by this antibody is located within the type III repeat 8 of FN. IHC analysis showed that this antibody strongly binds human-specific epitopes on repeat III8 of FN in neoplastic tissues. A murine urokinase-type plasminogen activator-conjugated mini-antibody version of the antibody was generated and utilized for in vivo tumor-targeting experiments in tumor-bearing mice expressing human B-FN using a radioiodinated mini-antibody. The distribution of the radioiodinated mini-antibody was assessed in tumor tissue and blood at different time points after injection, and the results showed rapid clearance from the bloodstream. Furthermore, the ratio of the percentage of injected dose per gram of tissue (%ID/g) in the tumor versus blood was higher than 10 in all cases, indicating high specificity for tumor tissue (Balza et al., 2009; PMID: 19479996). The reactivity of the scFv version of this antibody with wild-type and mutated fragments of the recombinant FN fragment EDB-III8 was examined via ELISA. It was found that the epitope of this antibody encompasses both E1329 on ED-B and D1385 on III8 and the simultaneous presence of both residues is required for the reaction; neither of the two residues alone is sufficient to ensure the interaction of this antibody with B-FN (Ventura et al., 2016; PMID: 26867013).

Western Blot, ELISA, ImmunoHistoChemistry, Other Application

Research Use Only

Monoclonal

C6

FN1

fibronectin 1

CIG; cold-insoluble globulin; ED-B; epididymis secretory sperm binding protein; fibronectin; FINC; FN; FNZ; GFND; GFND2; LETS; migration-stimulating factor; MSF; SMDCF

Mouse

IgG1

Fibronectin

-20°C,2°C to 8°C

12352203