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Immunohistochemical staining of human endometrium shows strong immunoreactivity in endometrial stroma and basement membrane of glandular cells.
Immunohistochemical staining of human endometrium shows strong immunoreactivity in endometrial stroma and basement membrane of glandular cells.
Immunohistochemical staining of human endometrium shows strong immunoreactivity in endometrial stroma and basement membrane of glandular cells.

Anti-FN1 Antibody

AMAB91223
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFN1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FN1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL3730
  • Conjugate
    Unconjugated
  • Gene ID2335
  • Target name
    FN1
  • Target description
    fibronectin 1
  • Target synonyms
    CIG; cold-insoluble globulin; ED-B; epididymis secretory sperm binding protein; fibronectin; FINC; FN; FNZ; GFND; GFND2; LETS; migration-stimulating factor; MSF; SMDCF
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP02751
  • Protein Name
    Fibronectin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FN1 using anti-FN1 antibody (A00564-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FN1 antigen affinity purified polyclonal antibody (Catalog # A00564-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FN1 at approximately 220-400 kDa. The expected band size for FN1 is at 272 kDa.
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