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Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.

Anti-FKBP15 Antibody

HPA007979
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFKBP15
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FKBP15 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23307
  • Target name
    FKBP15
  • Target description
    FKBP prolyl isomerase family member 15
  • Target synonyms
    FKBP133, KIAA0674, PPP1R76, FK506-binding protein 15, 133 kDa FK506-binding protein, 133 kDa FKBP, FK506 binding protein 15, 133kDa, FK506-binding protein 133kDa, FKBP prolyl isomerase 15, FKBP-133, FKBP-15, WAFL, WASP and FKBP-like protein, protein phosphatase 1, regulatory subunit 76
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5T1M5
  • Protein Name
    FK506-binding protein 15
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of FKBP135/FKBP15 using anti-FKBP135/FKBP15 antibody (A10447-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP135/FKBP15 antigen affinity purified polyclonal antibody (Catalog # A10447-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FKBP135/FKBP15 at approximately 160 kDa. The expected band size for FKBP135/FKBP15 is at 160 kDa.
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