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ProductsBack/FKBP15 Antibody (aa50-100)

FKBP15 Antibody (aa50-100)

LS-C285983
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetFKBP15
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FKBP15 Antibody (aa50-100)
  • Delivery Days Customer
    23
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    IP (2 - 10 µg/mg lysate), WB (1:2000 - 1:10000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23307
  • Target name
    FKBP15
  • Target description
    FKBP prolyl isomerase family member 15
  • Target synonyms
    FKBP133, KIAA0674, PPP1R76, FK506-binding protein 15, 133 kDa FK506-binding protein, 133 kDa FKBP, FK506 binding protein 15, 133kDa, FK506-binding protein 133kDa, FKBP prolyl isomerase 15, FKBP-133, FKBP-15, WAFL, WASP and FKBP-like protein, protein phosphatase 1, regulatory subunit 76
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FKBP135/FKBP15 using anti-FKBP135/FKBP15 antibody (A10447-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP135/FKBP15 antigen affinity purified polyclonal antibody (Catalog # A10447-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FKBP135/FKBP15 at approximately 160 kDa. The expected band size for FKBP135/FKBP15 is at 160 kDa.
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