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Anti-FKBP4 Antibody

Anti-FKBP4 Antibody

A30849
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-FKBP4 Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    >95%
  • Host
    Rabbit
  • Scientific Description
    Rabbit polyclonal antibody to FKBP4
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, PC-3 whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate All lanes: FKBP4 antibody at 3.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 59 kDa
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Figure 1. Western blot analysis of FKBP52/FKBP4 using anti-FKBP52/FKBP4 antibody (A02165-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP52/FKBP4 antigen affinity purified polyclonal antibody (Catalog # A02165-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FKBP52/FKBP4 at approximately 56-59 kDa. The expected band size for FKBP52/FKBP4 is at 52 kDa.
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