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Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, PC-3 whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate All lanes: FKBP4 antibody at 3.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 59 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, PC-3 whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate All lanes: FKBP4 antibody at 3.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 59 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, PC-3 whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate All lanes: FKBP4 antibody at 3.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 59 kDa

FKBP4 Antibody

CSB-PA008700EA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFKBP4
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Overview

  • Supplier
    Cusabio
  • Product Name
    FKBP4 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2288
  • Target name
    FKBP4
  • Target description
    FKBP prolyl isomerase 4
  • Target synonyms
    FKBP51, FKBP52, FKBP59, HBI, Hsp56, PPIase, p52, peptidyl-prolyl cis-trans isomerase FKBP4, FK506 binding protein 4, 59kDa, HSP binding immunophilin, T-cell FK506-binding protein, 59kD, peptidylprolyl cis-trans isomerase, rotamase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02790
  • Protein Name
    Peptidyl-prolyl cis-trans isomerase FKBP4
  • Scientific Description
    Immunophilin protein with PPIase and co-chaperone activities. Component of steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly. May have a protective role against oxidative stress in mitochondria.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FKBP52/FKBP4 using anti-FKBP52/FKBP4 antibody (A02165-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP52/FKBP4 antigen affinity purified polyclonal antibody (Catalog # A02165-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FKBP52/FKBP4 at approximately 56-59 kDa. The expected band size for FKBP52/FKBP4 is at 52 kDa.
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