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ProductsBack/Anti-FLNB [3F10]

Anti-FLNB [3F10]

AB04172-10.0
Absolute Antibody
ApplicationsELISA
Product group Antibodies
TargetFLNB
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-FLNB [3F10]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The specificity of the original format of the antibody was confirmed by ELISA analysis. The binding kinetics of the antibody to the full length FLNA protein was measured by BLI (Kd= 1 nM) (WO2018085441A1).
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3F10
  • Gene ID2317
  • Target name
    FLNB
  • Target description
    filamin B
  • Target synonyms
    ABP-278, ABP-280, AOI, FH1, FLN-B, FLN1L, LRS1, SCT, TABP, TAP, filamin-B, ABP-280 homolog, Larsen syndrome 1 (autosomal dominant), actin binding protein 278, actin-binding-like protein, beta-filamin, filamin B, beta, filamin homolog 1, filamin-3, thyroid autoantigen
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDO75369
  • Protein Name
    Filamin-B
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Filamin B/FLNB using anti-Filamin B/FLNB antibody (A02562-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Filamin B/FLNB antigen affinity purified polyclonal antibody (Catalog # A02562-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Filamin B/FLNB at approximately 278 kDa. The expected band size for Filamin B/FLNB is at 278 kDa.
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