Figure 4. IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (PB9623). Flotillin 2 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Flotillin 2 Antibody (PB9623) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of Flotillin 2 using anti-Flotillin 2 antibody (PB9623). Flotillin 2 was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Flotillin 2 Antibody (PB9623) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Anti-Flotillin 2/FLOT2 Antibody Picoband(r)
PB9623-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFLOT2
Overview
- SupplierBoster Bio
- Product NameAnti-Flotillin 2/FLOT2 Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID2319
- Target nameFLOT2
- Target descriptionflotillin 2
- Target synonymsECS-1, ECS1, ESA, ESA1, M17S1, flotillin-2, Flotillin 2 (epidermal surface antigen 1), epidermal surface antigen, membrane component chromosome 17 surface marker 1, membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1)
- HostRabbit
- IsotypeIgG
- Protein IDQ14254
- Protein NameFlotillin-2
- Scientific DescriptionBoster Bio Anti-Flotillin 2/FLOT2 Antibody Picoband® catalog # PB9623. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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