Anti-FLT3 [D3-D4 (D4-3)]

Absolute Antibody

Anti-FLT3 [D3-D4 (D4-3)]

9

The antibody binds an epitope within domain D4 of human FLT3.

The clone was shown to selectively bind to human FLT3 with high affinity and to block FL binding. The ability of the antibody to bind the receptor was examined by ELISA (EC50= 0.2 nM). The ability of the antibody to block receptor-ligand binding was examined in a FL binding competition ELISA, the antibody blocked the binding of FL to FLT3 with a IC50 within the range of 7 nM. The clone binds FLT3 with an affinity of 0.27 nM as determined by surface plasmon resonance (SPR). The in vivo efficacy of the clone was determined in SEM-K2 cells, a human ALL cell line expressing wild type FLT3. In comparison to human IgG, the clone treatment all significantly prolonged survival in the SEM-K2 leukemia model (US8071099B2).

Flow Cytometry, ELISA, Other Application

Research Use Only

Monoclonal

D3-D4 (D4-3)

FLT3

fms related receptor tyrosine kinase 3

CD135; CD135 antigen; fetal liver kinase 2; FL cytokine receptor; FLK2; FLK-2; fms related tyrosine kinase 3; fms-like tyrosine kinase 3; growth factor receptor tyrosine kinase type III; receptor-type tyrosine-protein kinase FLT3; stem cell tyrosine kinase 1; STK1

Human

IgG1

Receptor-type tyrosine-protein kinase FLT3

This reformatted human antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.

Human

-20°C,2°C to 8°C

12352203