Anti-FLT3 [EB10]

Absolute Antibody

Anti-FLT3 [EB10]

7

The antibody binds an epitope within domain D4 of human FLT3.

The clone was shown to selectively bind to human FLT3 with high affinity, block FL binding and mediated potent anti-tumor activity in xenograft model by a mechanism involving activation of immune effector function. The ability of the antibody to bind the receptor was examined by ELISA (EC50 within the range 0.5-1 nM). The ability of the antibody to block receptor-ligand binding was examined in a FL binding competition ELISA, the antibody blocked the binding of FL to FLT3 with a IC50 within the range of 0.5-1 nM. The clone binds FLT3 with an affinity of 0.158 nM as determined by surface plasmon resonance (SPR). Flow cytometric analysis showed that the antibody bound to the wild-type FLT-3 expressed on EOL-1 cells and also to ITD-mutant FLT3 expressed on BaF3-ITD cells (US8071099B2).

Flow Cytometry, ELISA, Other Application

ELISA; FC; SPR

Research Use Only

Monoclonal

EB10

FLT3

fms related receptor tyrosine kinase 3

CD135; CD135 antigen; fetal liver kinase 2; FL cytokine receptor; FLK2; FLK-2; fms related tyrosine kinase 3; fms-like tyrosine kinase 3; growth factor receptor tyrosine kinase type III; receptor-type tyrosine-protein kinase FLT3; stem cell tyrosine kinase 1; STK1

Human

IgG1

Receptor-type tyrosine-protein kinase FLT3

This reformatted human antibody was made using the variable domain sequences of the original Human Fab format for improved compatibility with existing reagents assays and techniques.

Human

Human

The original Fab antibody was isolated from a human Fab phage display by selection of a high affinity antibody binding to the human FLT3.

-20°C,2°C to 8°C

12352203