Figure 1. Western blot analysis of Flt3 / CD135 using anti-Flt3 / CD135 antibody (A00188-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Flt3 / CD135 antigen affinity purified polyclonal antibody (Catalog # A00188-4) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Flt3 / CD135 at approximately 160KD. The expected band size for Flt3 / CD135 is at 113KD.
Figure 1. Western blot analysis of Flt3 / CD135 using anti-Flt3 / CD135 antibody (A00188-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Flt3 / CD135 antigen affinity purified polyclonal antibody (Catalog # A00188-4) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Flt3 / CD135 at approximately 160KD. The expected band size for Flt3 / CD135 is at 113KD.
Anti-Flt3/CD135 Antibody Picoband(r)
A00188-4-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
TargetFlt3
Overview
- SupplierBoster Bio
- Product NameAnti-Flt3/CD135 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, Western Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID14255
- Target nameFlt3
- Target descriptionFMS-like tyrosine kinase 3
- Target synonymsB230315G04; CD135; fetal liver kinase 2; FL cytokine receptor; Flk; Flk-; Flk2; Flk-2; Flt-; Flt-3; Ly72; receptor-type tyrosine-protein kinase FLT3; tyrosine-protein kinase FLT3; tyrosine-protein kinase receptor flk-2; wmf; wmfl
- HostRabbit
- IsotypeIgG
- Protein IDQ00342
- Protein NameReceptor-type tyrosine-protein kinase FLT3
- Scientific DescriptionBoster Bio Anti-Flt3/CD135 Antibody Picoband® catalog # A00188-4. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
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