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FMS Like Tyrosine Kinase 3 (Flt3) Polyclonal Antibody

CAU28890
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetFlt3
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Overview

  • Supplier
    Biomatik
  • Product Name
    FMS Like Tyrosine Kinase 3 (Flt3) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID14255
  • Target name
    Flt3
  • Target description
    FMS-like tyrosine kinase 3
  • Target synonyms
    B230315G04, CD135, Flk-2, Flk2, Flt-3, Ly72, wmfl, receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor, fetal liver kinase 2, tyrosine-protein kinase FLT3, tyrosine-protein kinase receptor flk-2
  • Host
    Rabbit
  • Protein IDQ00342
  • Protein Name
    Receptor-type tyrosine-protein kinase FLT3
  • Scientific Description
    The FMS Like Tyrosine Kinase 3 (Flt3) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Mouse, Rat
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Flt3 / CD135 using anti-Flt3 / CD135 antibody (A00188-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Flt3 / CD135 antigen affinity purified polyclonal antibody (Catalog # A00188-4) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Flt3 / CD135 at approximately 160KD. The expected band size for Flt3 / CD135 is at 113KD.
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