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Immunohistochemical staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.

Anti-FOS Antibody

HPA018531
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFOS
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FOS
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2353
  • Target name
    FOS
  • Target description
    Fos proto-oncogene, AP-1 transcription factor subunit
  • Target synonyms
    AP-1, C-FOS, p55, protein c-Fos, FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS), FBJ murine osteosarcoma viral oncogene homolog, Fos proto-oncogene, AP-1 trancription factor subunit, G0/G1 switch regulatory protein 7, activator protein 1, cellular oncogene c-fos, proto-oncogene c-Fos, transcription factor AP-1 subunit c-Fos
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01100
  • Protein Name
    Proto-oncogene c-Fos
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of c-Fos/FOS using anti-c-Fos/FOS antibody (A00297-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Sh-sy5y whole cell lysates, Lane 4: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Fos/FOS antigen affinity purified polyclonal antibody (Catalog # A00297-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Fos/FOS at approximately 50KD. The expected band size for c-Fos/FOS is at 50KD.
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