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Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm.
Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm.
Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm.

Anti-FOXF1-25ul

HPA028886
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FOXF1-25ul
  • Delivery Days Customer
    6
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    ICC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12946
  • Protein Name
    Forkhead box protein F1
  • Scientific Description
    Rabbit Polyclonal Anti-FOXF1 Antibody against Human forkhead box F1. Validated for Immunofluorescence and Western Blot
  • Reactivity
    Human
  • Storage Instruction
    Store at +4°C for short term storage. Long time storage is recommended at -20°C.
  • UNSPSC
    12352203

Datasheet

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MSDS

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Figure 1. Western blot analysis of FOXF1 using anti-FOXF1 antibody (A03563-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXF1 antigen affinity purified polyclonal antibody (Catalog # A03563-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXF1 at approximately 38-40KD. The expected band size for FOXF1 is at 40KD.
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