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ProductsBack/FOXF1 Recombinant Antibody, AbBy Fluor-647 Conjugated

FOXF1 Recombinant Antibody, AbBy Fluor-647 Conjugated

BSM-62250R-BF647
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetFOXF1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    FOXF1 Recombinant Antibody, AbBy Fluor-647 Conjugated
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Other Conjugate
  • Gene ID2294
  • Target name
    FOXF1
  • Target description
    forkhead box F1
  • Target synonyms
    ACDMPV; FKHL5; forkhead box protein F1; Forkhead, drosophila, homolog-like 5; forkhead-related activator 1; forkhead-related protein FKHL5; forkhead-related transcription factor 1; FREAC1; FREAC-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12946
  • Protein Name
    Forkhead box protein F1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FOXF1 using anti-FOXF1 antibody (A03563-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse SP20 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXF1 antigen affinity purified polyclonal antibody (Catalog # A03563-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXF1 at approximately 38-40KD. The expected band size for FOXF1 is at 40KD.
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