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Western blot analysis in human cell line HEK 293 and human cell line A-431.
Western blot analysis in human cell line HEK 293 and human cell line A-431.
Western blot analysis in human cell line HEK 293 and human cell line A-431.

Anti-FOXO1 Antibody

AMAB91844
Atlas Antibodies
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetFOXO1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FOXO1
  • Delivery Days Customer
    12
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL13278
  • Conjugate
    Unconjugated
  • Gene ID2308
  • Target name
    FOXO1
  • Target description
    forkhead box O1
  • Target synonyms
    FKH1, FKHR, FOXO1A, forkhead box protein O1, forkhead box protein O1A, forkhead, Drosophila, homolog of, in rhabdomyosarcoma
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ12778
  • Protein Name
    Forkhead box protein O1
  • Scientific Description
    Synthetic Peptide
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FOXO1A using anti-FOXO1A antibody (PB9195). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human FOXO1A protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXO1A antigen affinity purified polyclonal antibody (Catalog # PB9195) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXO1A at approximately 39 kDa. The expected band size for FOXO1A is at 39 kDa.
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