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FOXO1 antibody detects FOXO1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract 7.5 % SDS-PAGE FOXO1 antibody (GTX110724) dilution: 1:500
FOXO1 antibody detects FOXO1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract 7.5 % SDS-PAGE FOXO1 antibody (GTX110724) dilution: 1:500
FOXO1 antibody detects FOXO1 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract 7.5 % SDS-PAGE FOXO1 antibody (GTX110724) dilution: 1:500

FOXO1 antibody

GTX110724
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mammals, Xenopus
TargetFOXO1
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Overview

  • Supplier
    GeneTex
  • Product Name
    FOXO1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2308
  • Target name
    FOXO1
  • Target description
    forkhead box O1
  • Target synonyms
    FKH1, FKHR, FOXO1A, forkhead box protein O1, forkhead box protein O1A, forkhead, Drosophila, homolog of, in rhabdomyosarcoma
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12778
  • Protein Name
    Forkhead box protein O1
  • Scientific Description
    This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq]
  • Reactivity
    Human, Mammals, Xenopus
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FOXO1A using anti-FOXO1A antibody (PB9195). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human FOXO1A protein 0.5 ng. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXO1A antigen affinity purified polyclonal antibody (Catalog # PB9195) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXO1A at approximately 39 kDa. The expected band size for FOXO1A is at 39 kDa.
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