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Immunohistochemical staining of human anaplastic oligodendroglioma shows strong nuclear positivity in neurons.
Immunohistochemical staining of human anaplastic oligodendroglioma shows strong nuclear positivity in neurons.
Immunohistochemical staining of human anaplastic oligodendroglioma shows strong nuclear positivity in neurons.

Anti-FOXO3 Antibody

AMAB91872
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFOXO3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FOXO3
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CL13589
  • Conjugate
    Unconjugated
  • Gene ID2309
  • Target name
    FOXO3
  • Target description
    forkhead box O3
  • Target synonyms
    AF6q21, FKHRL1, FKHRL1P2, FOXO2, FOXO3A, forkhead box protein O3, FOXO3A-, forkhead box O3A, forkhead homolog (rhabdomyosarcoma) like 1, forkhead in rhabdomyosarcoma-like 1, forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDO43524
  • Protein Name
    Forkhead box protein O3
  • Scientific Description
    Synthetic Peptide
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FOXO3A/FOXO3 using anti-FOXO3A/FOXO3 antibody (A00252-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXO3A/FOXO3 antigen affinity purified polyclonal antibody (Catalog # A00252-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXO3A/FOXO3 at approximately 90 kDa. The expected band size for FOXO3A/FOXO3 is at 71,80-100 kDa.
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