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Anti-FOXP1 [RAB-S37]

Ab01718-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA
Product group Antibodies
TargetFOXP1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-FOXP1 [RAB-S37]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody has been used and recommended for the following methods: ELISA; spiked-IP; IF; FC.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA
  • Applications Supplier
    ELISA; spiked-IP; IF; FC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RAB-S37
  • Gene ID27086
  • Target name
    FOXP1
  • Target description
    forkhead box P1
  • Target synonyms
    12CC4, HSPC215, MFH, QRF1, hFKH1B, forkhead box protein P1, fork head-related protein like B, glutamine-rich factor 1, mac-1-regulated forkhead
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9H334
  • Protein Name
    Forkhead box protein P1
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was obtained by recombinant antibody (rAb) phage display recognizing FOXP1 protein under non-denaturing conditions.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FOXP1 using anti-FOXP1 antibody (A00723). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human MCF-7whole cell lysates, Lane 2: monkey COS-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXP1 antigen affinity purified polyclonal antibody (Catalog # A00723) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXP1 at approximately 82KD. The expected band size for FOXP1 is at 82KD.
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