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Western Blot analysis of K562 cells using FOXP1 Polyclonal Antibody
Western Blot analysis of K562 cells using FOXP1 Polyclonal Antibody
Western Blot analysis of K562 cells using FOXP1 Polyclonal Antibody

FOXP1 Antibody

CSB-PA005143
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFOXP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    FOXP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID27086
  • Target name
    FOXP1
  • Target description
    forkhead box P1
  • Target synonyms
    12CC4; fork head-related protein like B; forkhead box protein P1; glutamine-rich factor 1; hFKH1B; HSPC215; mac-1-regulated forkhead; MFH; QRF1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H334
  • Protein Name
    Forkhead box protein P1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FOXP1 using anti-FOXP1 antibody (A00723). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human MCF-7whole cell lysates, Lane 2: monkey COS-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXP1 antigen affinity purified polyclonal antibody (Catalog # A00723) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXP1 at approximately 82KD. The expected band size for FOXP1 is at 82KD.
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