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Immunofluorescent staining of human cell line RT4 shows localization to nucleus.
Immunofluorescent staining of human cell line RT4 shows localization to nucleus.
Immunofluorescent staining of human cell line RT4 shows localization to nucleus.

Anti-FRK Antibody

HPA030479
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetFRK
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-FRK
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2444
  • Target name
    FRK
  • Target description
    fyn related Src family tyrosine kinase
  • Target synonyms
    GTK, PTK5, RAK, tyrosine-protein kinase FRK, PTK5 protein tyrosine kinase 5, fyn-related kinase, nuclear tyrosine protein kinase RAK, protein-tyrosine kinase 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42685
  • Protein Name
    Tyrosine-protein kinase FRK
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of FRK using anti-FRK antibody (A04075-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRK antigen affinity purified polyclonal antibody (Catalog # A04075-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRK at approximately 58 kDa. The expected band size for FRK is at 58 kDa.
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