More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PTK5/FRK Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PTK5 Polyclonal Antibody, Unconjugated (bs-6382R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PTK5 Polyclonal Antibody, Unconjugated (bs-6382R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PTK5 Polyclonal Antibody, Unconjugated (bs-6382R) at 1:200 overnight at 4°C, DAB staining.

PTK5/FRK Polyclonal Antibody

BS-6382R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetFRK
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    PTK5 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2444
  • Target name
    FRK
  • Target description
    fyn related Src family tyrosine kinase
  • Target synonyms
    fyn-related kinase; GTK; nuclear tyrosine protein kinase RAK; protein-tyrosine kinase 5; PTK5; PTK5 protein tyrosine kinase 5; RAK; tyrosine-protein kinase FRK
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Biomatik
Fyn Related Kinase (FRK) Polyclonal AntibodyCAU22834
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetFRK
  • SizePrice
Product group Antibodies
RayBiotech
Anti-FRK Antibody102-26015
ApplicationsWestern Blot
TargetFRK
  • SizePrice
Anti-FRK Antibody
Product group Antibodies
Antibodies.com
Anti-FRK AntibodyA95485
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
MedChem Express
FRK Antibody (YA4017, Mouse)HY-P84320
TargetFRK
  • SizePrice
Western Blot analysis of NIH-3T3 K562 Jurkat cells using Rak Polyclonal Antibody
Product group Antibodies
Cusabio
FRK AntibodyCSB-PA003934
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetFRK
  • SizePrice
Immunofluorescent staining of human cell line RT4 shows localization to nucleus.
Product group Antibodies
Atlas Antibodies
Anti-FRK AntibodyHPA030479
ApplicationsImmunoCytoChemistry
TargetFRK
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
FRK AntibodyLS-C401692
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetFRK
  • SizePrice
Figure 1. Western blot analysis of FRK using anti-FRK antibody (A04075-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRK antigen affinity purified polyclonal antibody (Catalog # A04075-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRK at approximately 58 kDa. The expected band size for FRK is at 58 kDa.
Product group Antibodies
Boster Bio
Anti-FRK Antibody Picoband(r)A04075-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetFRK
  • SizePrice
Product group Antibodies
GeneTex
FRK antibodyGTX106156
ApplicationsWestern Blot
TargetFRK
  • SizePrice