Anti-FSH receptor [9H11]

Absolute Antibody

Anti-FSH receptor [9H11]

9

This antibody binds human and mice FSH receptor. FSH receptor is a G protein-coupled receptor for follitropin, the follicle-stimulating hormone. Through cAMP production activates the downstream PI3K-AKT and ERK1/ERK2 signaling pathways.

To test if the antibody dependent cellular cytotoxicity elicited by the mouse version of this antibody translated into an in vivo anti-tumor effect, mice were challenged with IDS -Defl29/Vegf-a-Fshr and treated with the mouse version of this antibody. The antibody was able to delay tumor progression. To further characterize the antibody its ability to bind cells expressing human and mouse FHSR, flow cytometry was performed using the mouse version of this antibody on A20 cells, ID8- Defl29/Vegf-a-Fshr cells and human ovarian cancer cell lines like Caov3, OVCAR3, TOV21 G and PC3 with physiological levels of FSHR. Isotype analysis of the antibody was done using ELISA. Finally immunofluorescence was performed on 293T cells transduced with human or murine FSHR using the mouse version of this antibody (WO2020033797).

Flow Cytometry, ImmunoFluorescence, ELISA

Research Use Only

Monoclonal

9H11

FSHR

follicle stimulating hormone receptor

follicle-stimulating hormone receptor; follitropin receptor; FSH receptor; FSHR1; FSHRO; LGR1; ODG1

Human

IgG1

Follicle-stimulating hormone receptor

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Human, Mouse

-20°C,2°C to 8°C

12352203